Organic Value Recovery Solutions LLC © Organic Value Recovery Solutions 2010 © Organic Value Recovery Solutions 2010 Book of Abstracts, III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009), Lisbon (Portugal), 2-4 December 2009 Hydrolytic biocatalysts isolated from intestinal microbiome in black soldier fly larvae, Hermetia illucens Chang-Muk Lee1, Bit Narae Yoon1, Soo-Jin Kim2, Ji-Young Choi3, Dong Gwan Kim1,5, Han-Chul Kang1, Yun-Soo Yeo2, Bon-Sung Koo4, Sang-Hong Yoon1 1Department of Agricultural Bio-resources, 2National Agrobiodiversity Center, 3Department of Agricultural Biology, 4Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-857, Korea 5Research Center for Drugs, Konkuk University, Seoul 143-701, Korea Black soldier fly, Hermetia illucens L., is a common colonizer of animal wastes. In its life cycle, larvae are voracious feeders of organic material, and may thus be used in simple engineering systems to convert large amount of organic waste into protein-rich biomass. In an effort to identify contributing biocatalysts at the prepupal stage, we have constructed a metagenomic fosmid library of an average insert size of ~30kb using the larval intestinal microbiome. To detect hydrolytic enzymes encoded by uncultured intestinal microorganisms that help H. illucens for processing daily food wastes, individual 92,544 metagenomic clones were subjected to substrate hydrolysis analysis. Initial screening of the libraries revealed 18 clones that use carboxymethyl cellulose as a sole carbon source. Additional screenings characterized four clones capable of starch degradation. Short-gun sequencing of the fosmid clones demonstrated putative cellulase and hydroxylase acting on cellulose or starch substrate. These results indicate that H. illucens hydrolyze manure nutrients in association with uncultured intestinal bacteria, enabling the fly to colonize successfully on a variety of animal wastes. Keywords Hermetia illucens; Metagenome; Hydrolysis; Biocatalyst